About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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Up to 3 values pKa1- pKa3 can be entered for each buffering component. It purigier also possible to view all three wavelengths simultaneously by turning the dial one step further only three digits. The peak is then collected in either one fraction volume or in several, using the Peak Fractionation Volume parameter.

To computer USB cable Fig The gradient is held at the value displayed and the pump continues to run. For example, click the Notes and Logbook purjfier to check the contents. The delay volume UV cell to fraction collector outlet is not set at system delivery because it depends on the length of your pueifier tubing. These valves can be used to accomplish the following functions: If they become full and overflow, liquid might penetrate the system causing a shortcircuit.

ÄKTApurifier – GE Healthcare Life Sciences

Also remove the desiccant bags. If a aktx flow cell optional is included in the flow path, it will be located after puridier conductivity cell. Original raw data curves can never be modified, renamed or deleted from a result file. All eluents commonly used in ion exchange, hydrophobic interaction, affinity and reversed phase chromatography can be mixed with a high degree of accuracy and reproducibility. If not, select View: Operation is subject to the following two conditions: An optional configuration consists of both hardware components and software instructions.


ÄKTApurifier User Guide – GE Healthcare Life Sciences |

This is a base with only one pKa. It is mainly used for file handling and administration.

Columns are snapped in place in column holders. Contact your local GE Healthcare representative for the most current information. To select access mode: Linear and step salt gradients can be run and pH can be used as a variable scouting parameter. Preparation Prepare the injection valve as follows: Only the pages set in the Start Protocol for the method will appear during the initialization.

The information selected here will be printed in the report. Please, refer to the Technical specifications for fuse data. UniNet-1 and Pjrifier, optional cabling installed. These examples can be used as a template for zwitter ions: Installed from factory at delivery.

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D In aita cases, the D parameter can reduce the oscillations introduced by a PI-regulator. Set the valve manually to this port. The faults and actions are listed as follows: Low eluent flow and noise as the piston moves 1 Replace or re-install the seal or membrane.

The new flow rate will be used until the end of the run or until a new flow rate instruction is reached in the method. Some packing lists are included in the boxes. Check if the signal is still noisy with aita.


ÄKTApurifier User Guide – GE Healthcare Life Sciences

Pump through at least 30 ml of buffer at the set pH to stabilize the pH. Check if there is too low concentration of buffer components causing low buffering capacity. Before starting to perform the described method below, make sure that the column itself is clean and does not generate too high back-pressure. The selection puifier on the modules can be set in one of three different access modes: A colon separates menu levels, thus File: For example, to change the flow rate: Connect the column between the injection valve, port 1, and the inlet port of the UV cell.

Please change the country on your profile in order to switch to another country store. The mixer function can also be checked by running the installation test. If so, replace pirifier connector. The tubing length from the flow restrictor to the outlet on the delivery arm is assumed to be mm. The run will start.

Active above the PressLevel msnual. If not, consult the Pump P User Manual for troubleshooting instructions. The ratios obtained should all fall within the intervals given in column 5. Refer to Section 6.