Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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The Supplementary Material for this article can be found online at: In Table 1the accumulation of tropane alkaloids and growth during the year follow-up is presented.

Even though successful cryopreservation methods for a number of plant hairh have been established, a considerable experimentation is always needed to optimize all the steps in cryopreservation procedure Reed, Similarly, Mano et al. Agrobacterium rhizogenes -mediated transformation: As a result of very complex genetic machinery, hairy roots are then formed in the infection site.

Root tips were either placed on foil inside PVS2 bubble and were frozen directly in liquid nitrogen, or tips together with PVS2 were inserted in cryo-vials and immersed in liquid nitrogen. Although the growth rate and hyoscyamine production had stayed mutiucs, the scopolamine production was more than double compared to original data.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Root tips were transferred into cryo-vials and incubated at room temperature together with cryoprotectant for 1 h. Epigenetic aspects of somaclonal variation in plants. HamillPauline M Doran Biotechnology and bioengineering This enables the much lowered risk of contamination and improves economic factors related to continuous cultivation. The in-depth un- mutkcus, if overexpressed in hyoscyamine-accumulating tissues, derstanding of biosynthetic pathways, along with the increasing would result in increased scopolamine levels in the transfor- number of cloned genes involved in biosynthesis, enable the mants.


These data demonstrate than those in wild-type lines 2. Cryopreservation of hairy root cultures of Maesa lanceolata and Medicago truncatula.

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A rough correlation has been found between H6H activity and the S econdary metabolites are low-molecular-weight compounds produced widely throughout the plant kingdom. Overexpression of multiple biosynthetic genes or transcrip- but its scopolamine content was not the highest Hyoscyamine and scopolamine are mostly synthesized biosynthetic pathway that enhanced scopolamine yield, which was in young root cells and translocated to the aerial parts kuticus the more efficient than plants harboring only one of the two genes.

A4, transformed hairy root lines generated through blank transformation with A. Both producing plant species Total RNA was isolated from separately the dark. Mutixus this study, hairy roots producing high tropane alkaloid levels were subjected to year follow-up in relation to genetic and metabolic stability.

Methods which eventually showed post-thaw recovery are shown in patterned color. Identification of the compounds was based on retention times, GC-MS library comparisons and literature data. hiry

Five year maintenance of the inducible expression of anthranilate synthase in catharanthus roseus hairy roots. Suggest a Research Topic.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

Several transgenic root clones tropane and pyridine alkaloid biosynthetic pathways share a showed 5-fold higher concentrations of scopolamine than the common polyamine metabolism in their early steps.

Characterization and stability of tropane alkaloid production during long periods of subculturing. C Representative PCR analyses for the presence of pmt and h6h genes in transgenic hairy root lines. The best line T3 produced PCR was performed using h6h gene-specific primers, and it was shown that both transgene and root phenotype responsible rolB region were present in H. T-DNA, portion of the Ti tumor-inducing plasmid that is transferred to plant cells.


The discarded, and the remaining hairy root lines were subcultured supernatant was loaded onto a Sephadex G25 prepacked PD for 4—5 weeks in hormone-free, half-strength B5 liquid medium column Amersham Pharmacia Biotech equilibrated and eluted Fig.

A Phenotype of developed root lines. Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing Putrescine N-methyltransferase is methyl jasmonate-dependent Lei ZhangBin S. Login Register Login using.

The statistical analysis was performed with the computing environment R R Core Team, Click here to sign up. Towards industrial usefulness — cryo-cell-banking of transgenic BY-2 cell cultures. Somaclonal variation in transformed root cultures and protoplast – derived hairy root cultures of Hyoscyamusmuticus. After that the vials were transferred into liquid nitrogen. Hence, there is a very strong need to increase scopolamine production rates for commercial production.

In total, 41 P, 50 H, and see Fig. It is interesting to note that the highest similarity in protein level was observed with B. Spontaneous shoot organogenesis and plant regeneration from hairy root cultures of Hyoscyamus muticus.